کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
599390 1454270 2015 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Chitosan coated nanostructured lipid carriers for brain delivery of proteins by intranasal administration
ترجمه فارسی عنوان
کیتوزان با استفاده از نانوساختار لاپید ها برای تحویل مغز از پروتئین ها با استفاده از تزریق داخل بینی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی شیمی کلوئیدی و سطحی
چکیده انگلیسی


• Design and optimization of a chitosan coated nanostructured lipid carrier (CS-NLC) with the capacity to reach the brain after being intranasally administered.
• The in vitro assays demonstrated the biocompatibility of the nanocarrier, and its cellular uptake by 16HBE14o- cells.
• No haemagglutination or haemolysis processes were observed when CS-NLCs were incubated with erythrocytes.
• No toxicity signals appeared in the nasal mucosa of mice after the administration of CS-NLCs.
• The biodistribution study of CS-NLC-DiR demonstrated an efficient brain delivery of the particles after intranasal administration.

The remarkable increase in the prevalence of neurodegenerative diseases has become a serious public health problem. Considering the lack of effective treatments to address these diseases and the difficulties in accessing the brain due to the blood–brain barrier (BBB), to attain a successful strategy to improve drug delivery to the brain, the administration route becomes a point of interest. The intranasal route provides a non-invasive method to bypass the BBB. Moreover, the development of new technologies for the protection and delivery of peptides is an interesting approach to consider. Thus, in this work, a suitable chitosan coated nanostructured lipid carrier (CS-NLC) formulation with the capacity to reach the brain after being intranasally administered was successfully developed and optimized. The optimal formulation displayed a particle size of 114 nm with a positive surface charge of +28 mV. The in vitro assays demonstrated the biocompatibility of the nanocarrier and its cellular uptake by 16HBE14o- cells. Furthermore, no haemagglutination or haemolysis processes were observed when the particles were incubated with erythrocytes, and no toxicity signals appeared in the nasal mucosa of mice after the administration of CS-NLCs. Finally, the biodistribution study of CS-NLC-DiR demonstrated an efficient brain delivery of the particles after intranasal administration. In conclusion, CS-NLC can be considered to be a safe and effective nanocarrier for nose-to-brain drug delivery; however, to obtain a higher concentration of the drug in the brain following intranasal administration, further modifications are warranted in the CS-NLC formulation.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Colloids and Surfaces B: Biointerfaces - Volume 134, 1 October 2015, Pages 304–313
نویسندگان
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