کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
599573 1454279 2015 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Enzyme-functionalized gold-coated magnetite nanoparticles as novel hybrid nanomaterials: Synthesis, purification and control of enzyme function by low-frequency magnetic field
ترجمه فارسی عنوان
نانوذرات مغناطیسی با طلا پوشش داده شده با آنزیم به عنوان نانومواد ترکیبی جدید: سنتز، تصفیه و کنترل عملکرد آنزیم با میدان مغناطیسی با فرکانس پایین
کلمات کلیدی
نانوذرات مغناطیسی با پوشش طلا، بی حرکت سازی آنزیم، پاکسازی، میدان مغناطیسی بدون حرارت بالا، مهار فعالیت کاتالیزوری آنزیم
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی شیمی کلوئیدی و سطحی
چکیده انگلیسی


• We have developed a procedure for the purification of the magnetite gold core–shell nanoparticles.
• We show that the enzyme activity decreases upon exposure of the NPs to the LF AC magnetic field.
• Activity of selected enzymes can be efficiently manipulated by a remote magnetic field.
• We demonstrate the effect of the spacer between NPs and enzyme on the kinetics of the enzyme reaction.

The possibility of remotely inducing a defined effect on NPs by means of electromagnetic radiation appears attractive. From a practical point of view, this effect opens horizons for remote control of drug release systems, as well as modulation of biochemical functions in cells. Gold-coated magnetite nanoparticles are perfect candidates for such application. Herein, we have successfully synthesized core–shell NPs having magnetite cores and gold shells modified with various sulphur containing ligands and developed a new, simple and robust procedure for the purification of the resulting nanoparticles. The carboxylic groups displayed at the surface of the NPs were utilized for NP conjugation with a model enzyme (ChT). In the present study, we report the effect of the low-frequency AC magnetic field on the catalytic activity of the immobilized ChT. We show that the enzyme activity decreases upon exposure of the NPs to the field.

Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Colloids and Surfaces B: Biointerfaces - Volume 125, 1 January 2015, Pages 104–109
نویسندگان
, , , , , , , , , , , ,