Molecularly imprinted poly(hydroxyethyl methacrylate) based cryogel for albumin depletion from human serum

• A newly synthesized functional monomer of MAPA was used for imprinting of HSA in precomplexation process.
• HSA imprinted PHEMA based cryogel was prepared to selectively removal of HSA out of human serum.
• HSA imprinted cryogel was optimized for using in the FPLC system.
• The efficiency of HSA depletion was illustrated in SDS-PAGE.

Macroporous cryogels imprinted with human serum albumin (HSA) have been prepared by copolymerization of 2-hydroxyethyl methacrylate with a functional co-monomer of N-methacryloyl-l-phenylalanine. The cryogels were used for the depletion of HSA from human serum. HSA-imprinted cryogels were prepared with gel fraction yields up to 90%, and their chemical structure, morphology and porosity were characterized by FTIR-spectroscopy, scanning electron microscopy, swelling studies and flow dynamics. Selective binding experiments were performed in the presence of competitive proteins like human transferrin and myoglobin. Albumin-imprinted cryogel column was optimized for fast protein liquid chromatography. Sodium-dodecyl sulfate polyacrylamide gel electrophoresis was used to show the efficiency of albumin depletion.

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