کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
600208 1454299 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Non-hazardous anticancerous and antibacterial colloidal ‘green’ silver nanoparticles
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی شیمی کلوئیدی و سطحی
پیش نمایش صفحه اول مقاله
Non-hazardous anticancerous and antibacterial colloidal ‘green’ silver nanoparticles
چکیده انگلیسی

Poly(ethylene glycol) stabilized colloidal silver nanoparticles were prepared using the reductive potency of the aqueous extract of Thuja occidentalis leaves under ambient conditions. The nanoparticles were well dispersed within a narrow size spectrum (7–14 nm) and displayed characteristic surface plasmon resonance peak at around 420 nm and Bragg's reflection planes of fcc structure. MTT assay revealed the dose-dependent cytocompatibility and toxicity of the nanoparticles with the L929 normal cell line. On the other hand, the antiproliferative action of the nanoparticles was evaluated on HeLa cell (cancerous cells) line. Fluorescence and phase contrast microscopic imaging indicated the appearance of multinucleate stages with aggregation and nuclear membrane disruption of the HeLa cells post treatment with the nanoparticles. The interaction at the prokaryotic level was also assessed via differential antibacterial efficacy against Staphylococcus aureus (MTCC 3160) and Escherichia coli (MTCC 40). Under these perspectives, it is also necessary to observe the environmental impact of the prepared silver nanoparticles. Hence, the dose dependent toxicity of silver nanoparticles was evaluated upon the earthworm species Eisenia fetida. Neither the survival nor the reproduction was affected by the addition of silver nanoparticles up to 1000 ppm. Thus these ‘green’ silver nanoparticles have promising potential as future materials.

Figure optionsDownload as PowerPoint slideHighlights
► Preparation of silver nanoparticles through greener route.
► Anticancerous but non-toxic to normal cell.
► Promising antibacterial efficacy to both gram positive and negative species.
► Promoting earthworm proliferation and growth.
► Environmentally benign, bioactive nanoparticles with potential future applications.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Colloids and Surfaces B: Biointerfaces - Volume 105, 1 May 2013, Pages 37–42
نویسندگان
, , , , , , , ,