کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
600349 1454301 2013 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Improvement of cell response of the poly(lactic-co-glycolic acid)/calcium phosphate cement composite scaffold with unidirectional pore structure by the surface immobilization of collagen via plasma treatment
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی شیمی کلوئیدی و سطحی
پیش نمایش صفحه اول مقاله
Improvement of cell response of the poly(lactic-co-glycolic acid)/calcium phosphate cement composite scaffold with unidirectional pore structure by the surface immobilization of collagen via plasma treatment
چکیده انگلیسی

In this study, calcium phosphate cement (CPC)-based scaffold with unidirectional lamellar pore structure was fabricated by unidirectional freeze casting. Poly(lactic-co-glycolic acid) (PLGA) was infiltrated into the CPC scaffold to improve its strength and toughness, which compromised the bioactivity and osteoconductivity of CPC. Collagen (Col) was immobilized on the pore surface of the PLGA/CPC scaffold to enhance the bioactivity of the scaffold using plasma treatment under the ammonia (NH3) atmosphere. The immobilization of collagen was characterized by infrared spectroscopy (ATR-FTIR), X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). Compared to the PLGA/CPC composite scaffold, the Col/PLGA/CPC composite scaffold had higher contact angle, porosity and water absorption, while the compressive strength of both scaffolds was comparable. Rat bone marrow mesenchymal stem cells (rMSCs) seeded on the Col/PLGA/CPC scaffold showed markedly improved cell seeding, attachment, proliferation and differentiation than those on the PLGA/CPC scaffold. These results suggest that the surface immobilization of collagen by plasma treatment can improve the bioactivity of the PLGA/CPC scaffold and the Col/PLGA/CPC composite scaffold is a promising candidate for bone tissue engineering.

Figure optionsDownload as PowerPoint slideHighlights
► PLGA/CPC scaffold with unidirectional lamellar pore structure was fabricated.
► Collagen was immobilized on the PLGA/CPC scaffold to enhance its bioactivity.
► The Col/PLGA/CPC scaffold showed markedly improved cell response.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Colloids and Surfaces B: Biointerfaces - Volume 103, 1 March 2013, Pages 209–216
نویسندگان
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