Rupture of single receptor–ligand bonds: A new insight into probability distribution function

Single molecule force spectroscopy is widely used to determine kinetic parameters of dissociation by analyzing bond rupture data obtained via applying mechanical force to cells, capsules, and beads that are attached to an intermolecular bond. The current analysis assumes that the intermolecular bond force is equal to the externally applied mechanical force. We confirm that viscous drag alone or in combination with cellular deformation resulting in viscoelasticity modulates bond force so that the instantaneous intermolecular bond force is not equivalent to the applied force. The bond force modulation leads to bond rupture time and force histograms that differ from those predicted by probability distribution function (PDF) using the current approach. A new methodology that accounts for bond force modulation in obtaining PDF is presented. The predicted histograms from the new methodology are in excellent agreement with the respective histograms obtained from Monte Carlo simulation.

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► The bond force modulation leads to different force and time histograms.
► We present a new theory that accounts for force modulation to obtain histograms.
► Predicted histograms agree with those obtained from the Monte Carlo simulation.