Tuning protein GlnB-Hs surface interaction with silicon: FTIR-ATR, AFM and XPS study

► Thin films of GlnB-Hs protein were prepared on hydrophilic and hydrophobic silicon. ► GlnB-Hs is adsorbed face-up on hydrophilic silicon deprotonating its residues. ► On hydrophobic silicon, GlnB-Hs forms filaments adopting side-on conformation. ► GlnB-Hs secondary structure was conserved after adsorption on both surfaces.