کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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600646 | 1454312 | 2012 | 9 صفحه PDF | دانلود رایگان |

Taking advantage of the large surface area that is covered with permanent positive charges of quaternary ammonium entities, this research aimed to develop environmentally friendly, organic antibacterial material from quaternized chitosan particles that may be applicable for biomedical devices, health and textile industries. The particles were formulated by ionic crosslinking of chitosan with tripolyphosphate followed by quaternization under heterogeneous conditions, via either direct methylation or reductive N-alkylation with a selected aldehyde followed by methylation. Sub-micron, spherical and positively charged quaternized chitosan particles were formed, as determined by 1H NMR, FT-IR, PCS and TEM analysis. Antibacterial activity tests, performed by viable cell (colony) counts, suggested that all quaternized chitosan particles exhibited superior antibacterial activity against the model Gram-positive bacteria, Staphylococcus aureus, as compared to the native chitosan particles at neutral pH. Only some quaternized chitosan particles, especially those having a high charge density and bearing large alkyl substituent groups, were capable of suppressing the growth of the model Gram-negative bacteria, Escherichia coli. The inhibitory efficiency of the quaternized chitosan particles was quantified in terms of the minimum inhibitory concentration (MIC). Damaging impact of the quaternized chitosan particles on the bacteria was also qualitatively determined by microscopic observation of the bacterial morphology.
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► Some surface-quaternized chitosan particles are more effective in inhibiting the bacterial growth than the quaternized chitosan solution.
► Quaternized chitosan particles prepared by heterogeneous route were more antibacterial than those prepared by homogeneous route.
► Only quaternized chitosan particles having high charge density and large alkyl groups were capable of suppressing the growth of E. coli.
Journal: Colloids and Surfaces B: Biointerfaces - Volume 92, 1 April 2012, Pages 121–129