IgG purification by bentonite–acrylamide–histidine microcomposite

In this work, a new microcomposite composed of bentonite, acrylamide and histidine, as a pseudospecific ligand, was synthesized by bulk polymerization. The aim of this study was to improve IgG adsorption capacity of bentonite by incorporating histidine. The surface areas of the bentonite and bentonite–acrylamide–histidine microcomposites were 33.4 and 1.42 m2/g, respectively. The amount of histidine was found to be 50 μmol/g bentonite via elemental analysis. Adsorption capacity was at the value of 100 mg/g from aqueous solution while adsorption capacity was 108 mg/g from human plasma with a purity of 90%. IgG biomolecules were able to be adsorbed and desorbed five times by using the same microcomposites without significant loss in their adsorption capacity.

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► A new support for pseudoaffinity (histidine) chromatography.
► The support was cross-linked bentonite–acrylamide composite.
► Adsorption capacity of bentonite was increased by using this new kind of microcomposite toward IgG.
► Immunoglobulin G purification was studied from human plasma.