Enhanced antimalarial activity of lumefantrine nanopowder prepared by wet-milling DYNO MILL technique

► Nanopowder formulation of lumefantrine (as nanosuspension) was developed to increase antimalarial efficacy of lumefantrine. ► In the present studies, lumefantrine was nano-sized using 'DYNO MILL' (wet milling process). Limited reports and literature is available for nano-sizing using DYNO MILL especially for drugs. ► Influence of formulation variables to obtain nano-sized, stable and redispersible lumefantrine nanopowder were discussed. ► Nano-sized lumefantrine powder and unprocessed lumefantrine powder were compared with respect to in vitro antimalarial activity against Plasmodium falciparum 3D7 strain and in vivo antimalarial activity against P. Yoelii nigeriensis (MDR).