Temperature-dependent structural changes on DDAB surfactant assemblies evidenced by energy dispersive X-ray diffraction and dynamic light scattering

► Above 55 °C silicon supported thin DDAB film undergoes a bilayer thinning process. ► From 55 °C DDAB-water vesicles show a corresponding decrease in size distribution. ► DDAB film cooled to 25 °C shows a water dependent gel-crystalline meta-stable phase. ► DDAB vesicles show reversible feature without metastable size distribution evidence. ► DDAB within DOPC lecithin vesicles allows for modulating their size by temperature.