کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
600836 1454310 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Measuring ligand–receptor binding events on polymeric surfaces with periodic wave patterns using liquid crystals
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی شیمی کلوئیدی و سطحی
پیش نمایش صفحه اول مقاله
Measuring ligand–receptor binding events on polymeric surfaces with periodic wave patterns using liquid crystals
چکیده انگلیسی

In this study, we measured ligand–receptor binding events on polymeric substrates with periodic nanostructures using liquid crystals (LCs). Periodic sinusoidal wave patterns were generated through the buckling of the poly-(dimethylsiloxane) (PDMS) substrate on a cylindrical surface followed by replicating the associated relief structures on a poly-(urethane acrylate) (PUA) surface, where a film of gold was deposited. Avidin was then covalently immobilized onto a gold surface decorated with carboxylic acid-terminated self-assembled monolayer via NHS/EDC chemistry. The optical response of the device showed that the orientation of nematic 4-cyano-4′-pentylbiphenyl (5CB) was parallel to the plane of the avidin surface. However, the formation of the avidin–biotin complexes disturbed the sinusoidal topographies of the surface, and induced a random orientation of LCs, which produced a distinctive change in the optical response. We also confirmed the specific ligand–receptor interactions using ellipsometry and atomic force microscopy (AFM). These results suggest that polymeric surfaces with continuous wavy features could be used to develop novel LC-based sensors for the detection of ligand–receptor binding events.

Figure optionsDownload as PowerPoint slideHighlights
► A label-free method to measure ligand–receptor binding events on a polymeric substrate with periodic nanostructures using liquid crystals.
► We observed a distinctive orientational transition of liquid crystals after binding of proteins.
► Polymeric substrates with conveniently fabricated continuous wavy features could be used to build LC-based sensors.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Colloids and Surfaces B: Biointerfaces - Volume 94, 1 June 2012, Pages 89–94
نویسندگان
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