کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
600903 1454311 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Sphingomyelin/phosphatidylcholine/cholesterol monolayers – analysis of the interactions in model membranes and Brewster Angle Microscopy experiments
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی شیمی کلوئیدی و سطحی
پیش نمایش صفحه اول مقاله
Sphingomyelin/phosphatidylcholine/cholesterol monolayers – analysis of the interactions in model membranes and Brewster Angle Microscopy experiments
چکیده انگلیسی

In this work the properties of two ternary sphingomyelin/phosphatidylcholine/cholesterol monolayers imitating erythrocyte membrane were studied at various content of sterol. Phosphatidylcholines chosen for experiments differ in the length of sn-1 saturated chain in the molecule (1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine–SOPC vs. 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine – POPC). Langmuir monolayer experiments combined with Brewster Angle Microscopy prove that for both investigated systems the most favorable effect of cholesterol appears at 30% of sterol in the film. However, the interactions between molecules at 50% of sterol are only slightly weaker as compared to those for 1:1:1 films. It was also found that only at higher sterol concentration appear differences in the ordering effect of cholesterol on the systems containing PC molecules of various length of sn-1 saturated chain. Although the differences in the properties of POPC versus SOPC-containing monolayers were found, similarities in the morphology of the respective systems and stoichiometry of thermodynamically the most favorable mixture allow one to conclude that both SM/POPC/Chol as well as SM/SOPC/Chol monolayer can be used to mimic raft systems.

Figure optionsDownload as PowerPoint slideHighlights
► Influence of cholesterol on sphingomyelin/phosphtidylcholines films was studied.
► The most favorable properties of the studied ternary systems occur at 30% of sterol.
► Phosphatidylcholines strongly determine the properties of the investigated films.
► Sterol/phosphatidylcholine forces contribute to the overall effect for ternary film.
► Differences in ordering effect of sterol on both films only at higher sterol range.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Colloids and Surfaces B: Biointerfaces - Volume 93, 1 May 2012, Pages 174–179
نویسندگان
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