Peptide derived from Pvfp-1 as bioadhesive on bio-inert surface

Surface property is one important characteristic of materials, especially for ones that are bio-inert but designed for bio-medical application. In this study, we designed a series of peptides and compared their capacities as bioadhesive to improve the surface bioactivity of bio-inert material. The peptides were designed according to the sequence of Perna viridis foot protein 1 (Pvfp-1), one of the Mfp-1s (mussel foot protein 1) which play key roles in wet adhesion of mussel byssus. And the Teflon (PTFE) was chosen as a model of bio-inert material. With adsorption, adhesion and coating analysis, it was found that peptide C2 (M) (derived from the non-repeating region of Pvfp-1, contains modified DOPA) has superior coating and adhesion abilities especially on the bio-inert surface of PTFE. After coating with peptide C2 (M), the cell adhesion and spreading of osteoblast MC3T3-E1 cells on PTFE were significantly improved compared with those on non-coated surface, and the peptide-coating did not show any cell toxicity. Therefore, peptide C2 (M) is effective for improving the bioactivity of bio-inert PTFE, and could be potentially used as a bioadhesive on other bio-inert materials for biomedical application. Moreover, this study also provided new insights in designing other peptide-based bioadhesive materials.

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► Peptide C2 (M) showed outstanding adsorption and adhesion ability especially on the bio-inert PTFE.
► DOPA amount and position, and the hydrophobicity of peptide could affect adsorption and adhesion.
► C2 (M) coated well on PTFE and improved cell adhesion and cell viability on PTFE and was non-toxic.
► C2 (M) improves the bio-activity of PTFE, and it would be used as bioadhesive on bio-inert materials.