کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
601000 | 879929 | 2011 | 6 صفحه PDF | دانلود رایگان |

A bio-ink for covalent deposition of thermostable, high affinity biotin-binding chimeric avidin onto sol–gel substrates was developed. The bio-ink was prepared from heterobifunctional crosslinker 6-maleimidohexanoic acid N-hydroxysuccinimide which was first reacted either with 3-aminopropyltriethoxysilane or 3-aminopropyldimethylethoxysilane to form silane linkers 6-maleimide-N-(3-(triethoxysilyl)propyl)hexanamide or -(ethoxydimethylsilyl)propyl)-hexanamide. C-terminal cysteine genetically engineered to chimeric avidin was reacted with the maleimide group of silane linker in methanol/PBS solution to form a suspension, which was printed on sol–gel modified PMMA film. Different concentrations of chimeric avidin and ratios between silane linkers were tested to find the best properties for the bio-ink to enable gravure or inkjet printing. Bio-ink prepared from 3-aminopropyltriethoxysilane was found to provide the highest amount of active immobilized chimeric avidin. The developed bio-ink was shown to be valuable for automated fabrication of avidin-functionalized polymer films.
Figure optionsDownload as PowerPoint slideHighlights
► Printable bio-ink to covalently immobilize protein onto sol–gel substrate.
► Bio-ink was prepared using heterobifunctional crosslinker to attach avidin to silane.
► Silane unit reacted with the surface to covalently immobilize avidin.
► Bio-ink was both gravure and inkjet printed.
► 3-Aminopropylsilane gave best results.
Journal: Colloids and Surfaces B: Biointerfaces - Volume 87, Issue 2, 15 October 2011, Pages 409–414