کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
601019 879930 2011 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Permeability of anti-fouling PEGylated surfaces probed by fluorescence correlation spectroscopy
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی شیمی کلوئیدی و سطحی
پیش نمایش صفحه اول مقاله
Permeability of anti-fouling PEGylated surfaces probed by fluorescence correlation spectroscopy
چکیده انگلیسی

The present work reports on in situ observations of the interaction of organic dye probe molecules and dye-labeled protein with different poly(ethylene glycol) (PEG) architectures (linear, dendron, and bottle brush). Fluorescence correlation spectroscopy (FCS) and single molecule event analysis were used to examine the nature and extent of probe–PEG interactions. The data support a sieve-like model in which size-exclusion principles determine the extent of probe–PEG interactions. Small probes are trapped by more dense PEG architectures and large probes interact more with less dense PEG surfaces. These results, and the tunable pore structure of the PEG dendrons employed in this work, suggest the viability of electrochemically-active materials for tunable surfaces.

Figure optionsDownload as PowerPoint slideHighlights
► Interactions of organic dye and dye-labeled protein with several PEG architectures.
► Linear, dendron, and bottle brush architectures studied.
► Sieve-like behavior of PEGs involving size-exclusion principles.
► Small probes trapped by dense PEG; large probes interact with sparse PEG.
► Dendrons allow variable PEG density, optimal for fabrication of tunable surfaces.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Colloids and Surfaces B: Biointerfaces - Volume 88, Issue 1, 1 November 2011, Pages 31–38
نویسندگان
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