کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
601077 879930 2011 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Silicon supported lipid–DNA thin film structures at varying temperature studied by energy dispersive X-ray diffraction and neutron reflectivity
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی شیمی کلوئیدی و سطحی
پیش نمایش صفحه اول مقاله
Silicon supported lipid–DNA thin film structures at varying temperature studied by energy dispersive X-ray diffraction and neutron reflectivity
چکیده انگلیسی

Non-viral gene transfection by means of lipid-based nanosystems, such as solid supported lipid assemblies, is often limited due to their lack of stability and the consequent loss of efficiency. Therefore not only a detailed thermo-lyotropic study of these DNA–lipid complexes is necessary to understand their interaction mechanisms, but it can also be considered as a first step in conceiving and developing new transfection biosystems.The aim of our study is a structural characterization of 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine (DOPC)–dimethyl-dioctadecyl-ammonium bromide (DDAB)–DNA complex at varying temperature using the energy dispersive X-ray diffraction (EDXD) and neutron reflectivity (NR) techniques.We have shown the formation of a novel thermo-lyotropic structure of DOPC/DDAB thin film self-organized in multi-lamellar planes on (1 0 0)-oriented silicon support by spin coating, thus enlightening its ability to include DNA strands. Our NR measurements indicate that the DOPC/DDAB/DNA complex forms temperature-dependent structures. At 65 °C and relative humidity of 100% DNA fragments are buried between single lamellar leaflets constituting the hydrocarbon core of the lipid bilayers. This finding supports the consistency of the hydrophobic interaction model, which implies that the coupling between lipid tails and hypo-hydrated DNA single strands could be the driving force of DNA–lipid complexation. Upon cooling to 25 °C, EDXD analysis points out that full-hydrated DOPC–DDAB–DNA can switch in a different metastable complex supposed to be driven by lipid heads–DNA electrostatic interaction. Thermotropic response analysis also clarifies that DOPC has a pivotal role in promoting the formation of our observed thermophylic silicon supported lipids–DNA assembly.

Figure optionsDownload as PowerPoint slideHighlights
► DOPC–DDAB fusion can form a new solid supported lamellar thermo-lyotropic structure.
► DOPC–DDAB mixture can form highly structured and thermal-dependent lipoplexes.
► At 65 °C DNA is trapped into the hydrophobic core of each lamellar DOPC–DDAB bilayer.
► Upon cooling to 25 °C the lipoplex switches to a different and metastable structure.
► DNA can affect the structural response of DDAB to varying temperature.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Colloids and Surfaces B: Biointerfaces - Volume 88, Issue 1, 1 November 2011, Pages 432–439
نویسندگان
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