کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
601080 | 879930 | 2011 | 8 صفحه PDF | دانلود رایگان |

An amphoteric copolymer brush of methacrylic acid (MA) and 2-(dimethylamino)ethyl methacrylate (DMAEMA) was prepared by reversible addition-fragmentation chain-transfer (RAFT) polymerization using both a free chain transfer agent (n-butylsulfanylthiocarbonylsulfanyl-2-methyl propionic acid) and a radical initiator (4,4′-azobis(4-cyanopentanoic acid)) covalently fixed to a glass substrate. An aqueous solution of the copolymer, Poly(MA-r-DMAEMA), which was simultaneously obtained in liquid phase, had a sufficiently small polydispersity in its molecular weight. The copolymer brush showed effective suppression of non-specific adsorption of bovine serum albumin and egg white lysozyme to the brush. In contrast, both negatively charged PolyMA and positively charged PolyDMAEMA brushes significantly adsorbed the proteins irrespective of their net charges. Upon ion beam irradiation, furthermore, a hollow space with a designed shape could be made on the glass substrate, and both HEK293 and HepG2 cells non-specifically adhered to the space, forming aggregates, while no adhesion to the non-treated area on the brush was observed. These results suggest that the amphoteric polymer brushes will be useful materials for biomedical applications.
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► A well-defined amphoteric copolymer brush of methacrylic acid and 2-(dimethylamino)ethyl methacrylate was prepared on a glass plate by RAFT polymerization.
► Non-specific adsorption of proteins to the brush was strongly suppressed.
► Both HEK293 and HepG2 cells did not adhere to the brush.
► Usefulness of the amphoteric polymer brush for biomedical applications was strongly suggested.
Journal: Colloids and Surfaces B: Biointerfaces - Volume 88, Issue 1, 1 November 2011, Pages 455–462