PVA and BSA stabilized silver nanoparticles based surface-enhanced plasmon resonance probes for protein detection

► To perform biosensing using nanoparticles in solution, the metal particles were coated with protein BSA and PVA as control stabilizer. ► Biocompatability study were carried out in mouse model and found no immunological and toxicity effect. The silver nanoparticles were distributed in all organs including brain. ► Functionalized silver nanoparticles with protein can be used as affinity probes in trapping antiBSA in this study or any molecule from aqueous solutions through SPR.