Nanoparticle immunoagglutination Rayleigh scatter assay to complement microparticle immunoagglutination Mie scatter assay in a microfluidic device

► First systematic comparison of nanoparticle and microparticle immunoagglutination assays with Rayleigh and Mie scattering detections, using both simulation and experiment. ► Size identification of immunoagglutinated particles for detecting Escherichia coli. ► Identification of complementary nature of nanoparticle-Rayleigh and microparticle-Mie scatter assays.