Interaction of an antidepressant buzepide methiodide with DNA immobilized on the glassy carbon electrode

In the present study, a DNA-biosensor was prepared using immobilization technique to investigate the interaction between an antidepressant, buzepide methiodide (BZP) and calf thymus DNA. BZP showed a quasireversible peak in Britton–Robinson (BR) buffer of pH 5 at bare glassy carbon electrode (GCE). At DNA modified GCE, the peak potential of BZP was observed to be shifted towards positive potential revealing intercalative mode of binding. The binding constant and stoichiometry between DNA and BZP are calculated to be 1.908 × 105 M−1 and 0.982, respectively. The spectroscopic techniques viz., spectrofluorescence and UV–vis absorption have also been employed to understand the interaction between BZP and DNA. The results serve as a reference for the interaction of BZP with DNA base pairs in the natural environment of living cells.

Figure optionsDownload as PowerPoint slideResearch highlights▶ Mechanism of interaction between DNA and buzepide methiodide has been studied. ▶ DNA-modified electrode was employed to study the binding. ▶ Positive shift in peak potential revealed intercalative mode of binding. ▶ Electrochemical and spectroscopic methods are useful to study the binding of a drug with DNA.