کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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601853 | 879955 | 2009 | 5 صفحه PDF | دانلود رایگان |
Biomolecule patterning is important due to its potential applications in biodevices, tissue engineering, and drug delivery. In this study, we developed a new method for a biomolecular patterning on poly(ɛ-caprolactone) (PCL) films based on ion implantation. Ion implantation on a PCL film surface resulted in the formation of carboxylic acid groups. The generated carboxylic acid groups were used for the covalent immobilization of amine-functionalized p-DNA, followed by hybridization with fluorescently tagged c-DNA. Biotin-amine was also covalently immobilized on the carboxylic acid generated PCL surfaces. Successful biotin-specific binding of streptavidin further confirmed the potential of this strategy for patterning of various biomolecules.
Journal: Colloids and Surfaces B: Biointerfaces - Volume 74, Issue 1, 1 November 2009, Pages 375–379