کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
601919 879958 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Microscale control over collagen gradient on poly(l-lactide) membrane surface for manipulating chondrocyte distribution
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی شیمی کلوئیدی و سطحی
پیش نمایش صفحه اول مقاله
Microscale control over collagen gradient on poly(l-lactide) membrane surface for manipulating chondrocyte distribution
چکیده انگلیسی

A collagen gradient was constructed to interrogate cell adhesion on a poly(l-lactide) (PLLA) membrane surface. Utilizing a microinfusion pump, gradients of amino groups were generated on the PLLA surface by an aminolysis method. Immobilization of collagen onto the gradient surfaces was performed by glutaraldehyde (GA) coupling to form the collagen gradients. The –NH2 and immobilized collagen density profiles on the PLLA membranes were quantitatively determined by ninhydrin and hydroproline (Hyp) analysis, respectively. By using fluorescein isothiocyanate (FITC) labeled collagen (FITC-Col), the profile of the as-prepared collagen gradient was directly monitored by a fluorescence microscope. The scanning force microscopy and water contact angle studies revealed that the morphology and wettability of the modified membranes changed progressively as a function of position along the gradient surface. Rabbit auricular chondrocytes were cultured on the collagen gradient membranes to test their cellular response. The attachment and spreading behaviors of the chondrocytes were dependent on the surface collagen density. These results indicate a surface on which the variation of collagen gradient strongly modifies the biological response of chondrocytes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Colloids and Surfaces B: Biointerfaces - Volume 67, Issue 2, 1 December 2008, Pages 210–215
نویسندگان
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