کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6020405 | 1580397 | 2014 | 7 صفحه PDF | دانلود رایگان |
- Treatment-to-target approach for RTX monitors CD19Â + B cells by flow cytometry (FC).
- Relapses occur despite the CD19 antigen remaining under the detection limit of FC.
- CD19 mRNA quantification is more sensitive than FC in detecting CD19Â + B cells.
- CD19 mRNA quantification can be applied to other antibodies against CD20Â + B cells.
We compared pre-amplification (PA) RT-PCR blood CD19 mRNA quantification with flow cytometry (FC), to personalize rituximab re-treatment in neuromyelitis optica spectrum disorders (NMOSDs) patients. 47 blood samples from 3 NMOSDs patients were studied. PA-RT-PCR quantified CD19 in all samples, and a positivity threshold was defined, whereas CD19Â + B cells were under threshold in 31/47 samples by FC. In all samples where CD19Â + B cells were above FC threshold, they resulted above the PA-RT-PCR threshold. CD19 mRNA was above threshold in 8 other samples, resulted negative by FC, and preceded the FC positivity in 7/8 samples by 1-3Â months, showing major sensitivity.
Journal: Journal of Neuroimmunology - Volume 277, Issues 1â2, 15 December 2014, Pages 127-133