کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
602186 | 879966 | 2008 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Electroformation in a flow chamber with solution exchange as a means of preparation of flaccid giant vesicles
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
شیمی کلوئیدی و سطحی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Electroformation in a flow chamber with solution exchange as a means of preparation of flaccid giant vesicles Electroformation in a flow chamber with solution exchange as a means of preparation of flaccid giant vesicles](/preview/png/602186.png)
چکیده انگلیسی
A recently described technique [Estes and Mayer, Biochim. Biophys. Acta 1712 (2005) 152–160] for the preparation of giant unilamellar vesicles (GUVs) in solutions with high ionic strength is examined. By observing a series of osmotic swellings followed by vesicle bursts upon a micropipette transfer of a single POPC GUV from a sucrose solution into an iso-osmolar glycerol solution, a value for the permeability of POPC membrane for glycerol, P=(2.09±0.82)×10−8P=(2.09±0.82)×10−8 m/s, has been obtained. Based on this result, an alternative mechanism is proposed for the observed exchange of vesicle interior. With modifications, the method of Estes and Mayer is then applied to preparation of flaccid GUVs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Colloids and Surfaces B: Biointerfaces - Volume 64, Issue 1, 15 June 2008, Pages 77–87
Journal: Colloids and Surfaces B: Biointerfaces - Volume 64, Issue 1, 15 June 2008, Pages 77–87
نویسندگان
Primož Peterlin, Vesna Arrigler,