کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
602193 879966 2008 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Preparation of mannan modified anionic PCL–PEG–PCL nanoparticles at one-step for bFGF antigen delivery to improve humoral immunity
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی شیمی کلوئیدی و سطحی
پیش نمایش صفحه اول مقاله
Preparation of mannan modified anionic PCL–PEG–PCL nanoparticles at one-step for bFGF antigen delivery to improve humoral immunity
چکیده انگلیسی

In this article, blank anionic poly(ɛ-caprolactone)-poly(ethylene glycol)-poly(ɛ-caprolactone) (PCEC) and anionic mannan modified PCEC (MPCEC) nanoparticles with nearly the same particle size and zeta potential were prepared by emulsion solvent evaporation method. Human basic fibroblast growth factor (bFGF) was absorbed onto anionic nanoparticles surface due to electrostatic interaction. The obtained bFGF-nanoparticles complexes were injected subcutaneously into C57BL/6 mice at 20 μg of bFGF/dose on week 0, 1, 2 and 3. The mice serum was collected on week 4, and bFGF-specific autoantibody total IgG, IgG1 and IgG2a titer in serum was determined by ELISA. The results indicated that the autoantibody IgG, IgG1 and IgG2a titer of the mice immunized by bFGF–MPCEC complexes were higher than that immunized by either bFGF–PCEC or bFGF–Alum. This phenomenon might be due to that mannan functionalized MPCEC nanoparticles could be targeted to dendritic cells (DCs) to improve humoral immunity. The prepared anionic mannan modified PCEC nanoparticles (MPCEC) might have great potential application in vaccine delivery systems.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Colloids and Surfaces B: Biointerfaces - Volume 64, Issue 1, 15 June 2008, Pages 135–139
نویسندگان
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