کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
602332 879972 2009 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Resonance light scattering spectral method for the determination of serum albumin with the interaction of neutral red–sodium dodecyl sulfonate
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی شیمی کلوئیدی و سطحی
پیش نمایش صفحه اول مقاله
Resonance light scattering spectral method for the determination of serum albumin with the interaction of neutral red–sodium dodecyl sulfonate
چکیده انگلیسی

Based on the enhancement of resonance light scattering (RLS) of serum albumin interaction with neutral red (NR) and sodium dodecyl sulfonate (SDS), a novel sensitive assay of serum albumins has been developed. Experimental conditions such as mixing sequence of reagents, pH, NR and SDS concentrations have been optimized. Linear relationships between the enhanced RLS intensity and the protein concentration were observed for bovine serum albumin (BSA) within the range of 0.01–5.0 μg mL−1 and human serum albumin (HAS) of 0.01–7.0 μg mL−1. The detection limits (S/N = 3) are 6.0 ng mL−1 for BSA and 5.0 ng mL−1 for HAS, respectively. The method was successfully applied to the determination of HSA in human blood plasma samples with recovery from 97.3 to 104.3%.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Colloids and Surfaces B: Biointerfaces - Volume 71, Issue 1, 1 June 2009, Pages 84–87
نویسندگان
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