کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
602615 1454318 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Two methods for glass surface modification and their application in protein immobilization
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی شیمی کلوئیدی و سطحی
پیش نمایش صفحه اول مقاله
Two methods for glass surface modification and their application in protein immobilization
چکیده انگلیسی

Protein immobilization is a crucial step in protein chip, biosensor, etc. Here, two methods to immobilize proteins on glass surface were analyzed, one is silanization method using 3-aminopropyltriethoxysilane (APTES), and the other is hydrophobin HFBI coating. The modified glass surfaces were characterized with X-ray photoelectron spectroscopy (XPS), water contact angle measurement (WCA) and immunoassay. The results of XPS and WCA illustrated that the surface property of glass can be changed by both the two methods. The following immunoassay using microcontact printing (μCP) verified that both methods could help protein immobilization effectively on glass slides. Compared with the amine treatment, it is concluded that hydrophobin self-assemblies is a simple and generic way for protein immobilization on glass slides, which has potential application in protein chips and biosensors.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Colloids and Surfaces B: Biointerfaces - Volume 60, Issue 2, 15 November 2007, Pages 243–249
نویسندگان
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