کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
602618 | 1454318 | 2007 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Glycerol facilitates the disaggregation of recombinant adeno-associated virus serotype 2 on mica surface
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
شیمی کلوئیدی و سطحی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Preparation of distributed virus on a solid substrate is a prerequisite for investigation of the properties and individualism of virus, while many previous studies showed that virus has a tendency to aggregate on solid substrates. In this communication, we report a novel approach by which well-separated recombinant adeno-associated virus serotype 2 (rAAV2) could be prepared on bare mica surface. The key technique in this approach is the addition of less than 3% (v/v) glycerol into the virus solution and subsequently deposition onto mica surface for the sample preparation. The possible mechanisms are also briefly discussed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Colloids and Surfaces B: Biointerfaces - Volume 60, Issue 2, 15 November 2007, Pages 264–267
Journal: Colloids and Surfaces B: Biointerfaces - Volume 60, Issue 2, 15 November 2007, Pages 264–267
نویسندگان
Peng Wang, Hai Li, Hai Jun Yang, Hua Bing Wang, Jun Hong Lü, Yi Zhang, Jun Hu,