کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
602784 | 879993 | 2007 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Preparation of latex reagents combined with IgM and its F(ab′)2 fragment from commercial ABO blood grouping reagent
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
شیمی کلوئیدی و سطحی
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![عکس صفحه اول مقاله: Preparation of latex reagents combined with IgM and its F(ab′)2 fragment from commercial ABO blood grouping reagent Preparation of latex reagents combined with IgM and its F(ab′)2 fragment from commercial ABO blood grouping reagent](/preview/png/602784.png)
چکیده انگلیسی
To achieve a rapid assay for ABO blood grouping using a latex reagent, two latex reagents were produced, one of which combined with mouse monoclonal immunoglobulin M (IgM) isolated from commercial ABO blood grouping reagent, and the other of which combined with its F(ab′)2 fragment prepared by cold pepsin digestion. The latex reagent adsorbing the F(ab′)2 fragment was able to detect the 1000-fold diluted saliva and provided much better sensitivity than that of IgM. This suggests that the difference in sensitivity between the two latex reagents is responsible for adsorption orientation of the antigen site on the latex particles. The new assay successfully completed the ABO blood grouping of cigarette ends within 30 min.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Colloids and Surfaces B: Biointerfaces - Volume 54, Issue 1, 15 January 2007, Pages 114–117
Journal: Colloids and Surfaces B: Biointerfaces - Volume 54, Issue 1, 15 January 2007, Pages 114–117
نویسندگان
Hiroaki Nakanishi, Takeshi Ohmori, Tomoko Akutsu, Koichi Sakurada,