The impact of “physiological correction” on functional connectivity analysis of pharmacological resting state fMRI

► The BOLD signal is sensitive to physiological noise near large vessels. ► Higher sensitivity to physiological signal in structures linked to autonomic control. ► Template-based dual-regression analysis, robust to effects of physiological noise. ► Group-level modeling of physiological rates changed the extent of morphine effects. ► Canonical cardiac and respiratory HRF filters not suitable in pharmacological tests.