Comparison of 2D and 3D single-shot ASL perfusion fMRI sequences

► 3D pCASL sequences increased perfusion spatial SNR by a factor of 2. ► The addition of BS to 3D readouts increased perfusion temporal SNR by a factor of 3. ► 2D EPI showed higher GM-to-WM CBF contrast ratio. ► Statistical power to detect functional activation was higher in 3D BS sequences. ► 3D BS single-shot sequences appear as the preferable readout choice in pCASL fMRI.