Quantification of receptor-ligand binding potential in sub-striatal domains using probabilistic and template regions of interest

► First use of probabilistic and template ROIs for Mawlawi sub-striatal regions. ► Probabilistic ROIs superior to template ROIs for binding potential quantification. ► ART spatial normalization produces superior results compared to SPM5. ► Probabilistic ROIs maintain statistical significance of results from manual ROIs. ► High quality probabilistic sub-striatal ROIs can be produced from ≥ 9 subjects.