کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6090389 1208572 2012 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Basic nutritional investigationAscorbic acid protects the diaphragm muscle against myonecrosis in mdx mice
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی غدد درون ریز، دیابت و متابولیسم
پیش نمایش صفحه اول مقاله
Basic nutritional investigationAscorbic acid protects the diaphragm muscle against myonecrosis in mdx mice
چکیده انگلیسی

ObjectiveOxidative stress contributes to myonecrosis in the dystrophin-deficient fibers of mdx mice and in Duchenne's muscular dystrophy. We examined the effects of ascorbic acid (AA), an antioxidant and free radical scavenger, on the dystrophic diaphragm muscle.MethodsMdx mice (14 d old) received AA for 14 d. Control mdx mice received saline. The muscle damage was visualized by the penetration of Evans blue dye into myofibers and the extent of inflammation was assessed by histologic analysis. Creatine kinase levels were measured for the biochemical evaluation of muscle fiber degeneration. The levels of tumor necrosis factor-α (a proinflammatory cytokine) and 4-hydroxynonenal (a marker of lipid peroxidation) were analyzed by immunoblotting.ResultsAscorbic acid decreased creatine kinase levels, myonecrosis, inflammation, and the levels of tumor necrosis factor-α and 4-hydroxynonenal.ConclusionThe present results suggest that AA plays a protective role in dystrophic muscle degeneration, possibly by decreasing reactive oxygen species, and support further investigations of AA as a potential therapy for dystrophinopathies.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Nutrition - Volume 28, Issue 6, June 2012, Pages 686-690
نویسندگان
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