کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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6090659 | 1208617 | 2008 | 7 صفحه PDF | دانلود رایگان |

ObjectiveTo establish the functional significance of endogenous peptide YY (PYY) and neuropeptide Y (NPY) as mediators of Y1 and Y2 absorptive tone in colonic mucosa.MethodsFunctional studies utilized descending colon from adult mice (wild type [WT] and peptide nulls) and ex vivo human colonic tissue (from patients undergoing bowel resections) measuring changes in basal ion transport. Peak increases in ion transport to Y1 or Y2 antagonists (BIBO3304 300 nM; BIIE0246 1 μM) were pooled (mean ± SEM) and compared using Student's unpaired t test (P ⤠0.05); some tissues received tetrodotoxin (TTX; 100 nM). PYY-positive L-cell numbers and NPY innervation were also compared.ResultsY1 and Y2 tones were present in human and WT mouse colon mucosa and only the latter was TTX sensitive. Y1 tone was unchanged in NPYâ/â but was â¼90% inhibited in PYYâ/â and abolished in PYYNPYâ/â colon mucosa. Y2 tone was reduced â¼50% in NPYâ/â and PYYâ/â tissues and was absent from PYYNPYâ/â colon. Residual Y2 and Y1 tones present in PYYâ/â mucosa were abolished by TTX. PYY ablation had no apparent effect on NPY innervation and PYY-positive cells were observed at the same frequency in NPYâ/â (56.7 ± 6.8 cells/section) and WT (55.0 ± 4.6 cells/section) colons. Double knockouts lacked PYY and NPY expression, but endocrine cells and enteric nerves were present with similar frequencies to those of WT mice.ConclusionEndogenous PYY mediates Y1 absorptive tone that is epithelial in origin, whereas Y2 tone is a combination of PYY and NPY mediation.
Journal: Nutrition - Volume 24, Issue 9, September 2008, Pages 900-906