کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6096906 | 1209877 | 2008 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The Characteristics of the Cell-Mediated Immune Response Identify Different Profiles of Occult Hepatitis B Virus Infection
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کلمات کلیدی
FITCPBMCsELISPOTCH-CCH-Binterferon - اینترفرونIFN - اینترفرون هاinterleukin - اینترلوکینperipheral blood mononuclear cells - سلول های تک هسته ای خون محیطیfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتenzyme-linked immunosorbent spot - نقطه آنزیم جذب آنزیمchronic hepatitis B - هپاتیت B مزمن، هپاتیت ب مزمنchronic hepatitis C - هپاتیت C مزمنpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمراز
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
بیماریهای گوارشی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Background& Aims: Hepatitis B virus (HBV) DNA detection in serum and/or in the liver of hepatitis B surface antigen (HBsAg)-negative patients with or without serologic markers of previous viral exposure is defined as occult HBV infection. Because the role of the adaptive response in keeping HBV replication under control in occult infection still is undefined, this study was performed to characterize the features of the HBV-specific T-cell response in this condition. Methods: HBV-specific T-cell frequency and function were tested ex vivo and after in vitro expansion in 32 HBsAg-negative patients undergoing diagnostic liver biopsy for chronic hepatitis C: 18 with occult HBV infection (11 anti-HBc-negative and 7 anti-HBc-positive patients) defined by the detection of intrahepatic HBV DNA by polymerase chain reaction; 14 without detectable intrahepatic HBV DNA (5 anti-HBc-positive and 9 anti-HBc-negative patients). Six patients with chronic hepatitis B and 7 HBsAg-inactive carriers were studied for comparison. Results: The presence or absence of serologic HBV markers defined 2 profiles of HBV-specific T-cell responses in occult infection. Anti-HBc-positive patients showed a T-cell response typical of protective memory, suggesting that this condition represents a resolved infection with immune-mediated virus control. In contrast, HBV-specific T cells in anti-HBc-negative patients did not readily expand and produce interferon-γ in vitro, suggesting the possibility of a low-dose infection insufficient to allow maturation of protective memory. Conclusions: Our results suggest different mechanisms of control of viral replication in seropositive and seronegative occult infections. Additional studies aimed at understanding possible different clinical implications are needed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gastroenterology - Volume 134, Issue 5, May 2008, Pages 1470-1481
Journal: Gastroenterology - Volume 134, Issue 5, May 2008, Pages 1470-1481
نویسندگان
Alessandro Zerbini, Massimo Pilli, Carolina Boni, Paola Fisicaro, Amalia Penna, Paola Di Vincenzo, Tiziana Giuberti, Alessandra Orlandini, Giuseppina Raffa, Teresa Pollicino, Giovanni Raimondo, Carlo Ferrari, Gabriele Missale,