کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6115413 | 1215903 | 2016 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Real time application of whole genome sequencing for outbreak investigation - What is an achievable turnaround time?
ترجمه فارسی عنوان
کاربرد زمان واقعی توالی کامل ژنوم برای بررسی شیوع بیماری - چه زمان تحویل قابل دستیابی است؟
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
میکروبیولوژی و بیوتکنولوژی کاربردی
چکیده انگلیسی
Whole genome sequencing (WGS) is increasingly employed in clinical settings, though few assessments of turnaround times (TAT) have been performed in real-time. In this study, WGS was used to investigate an unfolding outbreak of vancomycin resistant Enterococcus faecium (VRE) among 3 patients in the ICU of a tertiary care hospital. Including overnight culturing, a TAT of just 48.5 h for a comprehensive report was achievable using an Illumina Miseq benchtop sequencer. WGS revealed that isolates from patient 2 and 3 differed from that of patient 1 by a single nucleotide polymorphism (SNP), indicating nosocomial transmission. However, the unparalleled resolution provided by WGS suggested that nosocomial transmission involved two separate events from patient 1 to patient 2 and 3, and not a linear transmission suspected by the time line. Rapid TAT's are achievable using WGS in the clinical setting and can provide an unprecedented level of resolution for outbreak investigations.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Diagnostic Microbiology and Infectious Disease - Volume 85, Issue 3, July 2016, Pages 277-282
Journal: Diagnostic Microbiology and Infectious Disease - Volume 85, Issue 3, July 2016, Pages 277-282
نویسندگان
Patrick McGann, Jessica L. Bunin, Erik Snesrud, Seema Singh, Rosslyn Maybank, Ana C. Ong, Yoon I. Kwak, Scott Seronello, Robert J. Clifford, Mary Hinkle, Stephen Yamada, Jason Barnhill, Emil Lesho,