کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6115416 | 1215903 | 2016 | 26 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Simultaneous detection of Legionella species and L. anisa, L. bozemanii, L. longbeachae and L. micdadei using conserved primers and multiple probes in a multiplex real-time PCR assay
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کلمات کلیدی
LP1Legionella longbeachaeLegionella anisaLegionella micdadeiMultiplex real-time PCR - PCR زمان واقعی MultiplexLegionnaires' disease - بیماری لژیونیرLOD یا Limit of detection - حد تشخیصLegionella - لژیونلاLegionella pneumophila serogroup 1 - لژیونلا پنوموفیلای سرووگروپ 1limit of detection - محدودیت تشخیصCommunity-acquired pneumonia - پنومونی به دست آمده در جامعهCaP - کلاه لبه دار
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Legionnaires' disease is a severe respiratory disease that is estimated to cause between 8,000 and 18,000 hospitalizations each year, though the exact burden is unknown due to under-utilization of diagnostic testing. Although Legionella pneumophila is the most common species detected in clinical cases (80-90%), other species have also been reported to cause disease. However, little is known about Legionnaires' disease caused by these non-pneumophila species. We designed a multiplex real-time PCR assay for detection of all Legionella spp. and simultaneous specific identification of four clinically-relevant Legionella species, L. anisa, L. bozemanii, L. longbeachae, and L. micdadei, using 5â²-hydrolysis probe real-time PCR. The analytical sensitivity for detection of nucleic acid from each target species was â¤50 fg per reaction. We demonstrated the utility of this assay in spiked human sputum specimens. This assay could serve as a tool for understanding the scope and impact of non-pneumophila Legionella species in human disease.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Diagnostic Microbiology and Infectious Disease - Volume 85, Issue 3, July 2016, Pages 295-301
Journal: Diagnostic Microbiology and Infectious Disease - Volume 85, Issue 3, July 2016, Pages 295-301
نویسندگان
Kristen E. Cross, Jeffrey W. Mercante, Alvaro J. Benitez, Ellen W. Brown, Maureen H. Diaz, Jonas M. Winchell,