کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6115479 | 1215905 | 2016 | 21 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
NINA-LAMP compared to microscopy, RDT, and nested PCR for the detection of imported malaria
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موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
میکروبیولوژی و بیوتکنولوژی کاربردی
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![عکس صفحه اول مقاله: NINA-LAMP compared to microscopy, RDT, and nested PCR for the detection of imported malaria NINA-LAMP compared to microscopy, RDT, and nested PCR for the detection of imported malaria](/preview/png/6115479.png)
چکیده انگلیسی
Microscopy and field adaptable rapid diagnostic tests (RDTs) are not sensitive and specific in certain conditions such as poor training of microscopists, lack of electricity, or lower sensitivity in the detection of non-falciparum malaria. More sensitive point-of-care testing (POCT) would reduce delays in diagnosis and initiation of therapy. In the current study, we have evaluated the efficacy of noninstrumented nucleic acid amplification (NINA) coupled with loop-mediated isothermal amplification (LAMP) for detection of traveler's malaria (n = 140) in comparison with microscopy, nested PCR, and the only Food and Drug Administration-approved rapid diagnostic test. NINA-LAMP was 100% sensitive and 98.6% specific when compared to nested PCR. For non-falciparum detection, NINA-LAMP sensitivity was 100% sensitive compared to nested PCR, whereas RDT sensitivity was 71%. LAMP is highly sensitive and specific for symptomatic malaria diagnosis regardless of species.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Diagnostic Microbiology and Infectious Disease - Volume 85, Issue 2, June 2016, Pages 149-153
Journal: Diagnostic Microbiology and Infectious Disease - Volume 85, Issue 2, June 2016, Pages 149-153
نویسندگان
Abu Naser Mohon, Lydia Da-Yeong Lee, Abebe Genetu Bayih, Asongna Folefoc, Dylan Guelig, Robert A. Burton, Paul LaBarre, Wilson Chan, Bonnie Meatherall, Dylan R. Pillai,