کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6115995 1215942 2013 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Evaluation of the diagnostic value of fluorescent in situ hybridization in a rat model of bacterial pneumonia
ترجمه فارسی عنوان
بررسی ارزش تشخیصی هیبریداسیون موضعی فلورسنت در مدل موش پنومونی باکتریایی
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
چکیده انگلیسی
In severe nosocomial pneumonia, the pathogenic responsibility of bacteria isolated from airways is far from certain, and a lung biopsy is sometimes performed. However, detection and identification of pathogens are frequently unachieved. Here, we developed a protocol for direct visualization of bacteria within the lung tissue using fluorescent in situ hybridization (FISH) in a rat model of Acinetobacter baumannii pneumonia. The reference positive diagnosis of bacterial pneumonia was the presence of pathological signs of pneumonia associated with the proof of bacteria or bacterial PCR products into the parenchyma. By analysis of 122 sets of slices from 26 rats and using the eubacterial probe EUB-338, our results show that FISH reached a sensitivity and a diagnostic accuracy higher than that of optic microscopy (sensitivity: 96% versus 55.4% and diagnostic accuracy: 96.7% versus 66.4%), whereas both approaches had 100% specificity. FISH could be useful especially on negative biopsies from patients with suspected infectious pneumonia.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Diagnostic Microbiology and Infectious Disease - Volume 76, Issue 4, August 2013, Pages 425-431
نویسندگان
, , , , , , , , ,