A control over accessibility of immobilized enzymes through porous coating layer

We report immobilization of an enzyme by layer-by-layer (LbL) film deposition technique. All the enzyme layers, including the inner ones, contributed to the activity. We put-forwarded additional coating layers to protect the enzymes. To control the accessibility of the enzymes beneath the coating layer, pores have been introduced. Our results show controlled accessibility of immobilized enzymes in solid-state matrices.

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