کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6144163 1594892 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Novel lean type 2 diabetic rat model using gestational low-protein programming
ترجمه فارسی عنوان
مدل ریاضی دیابت نوع 2 ریشه با استفاده از برنامه ریزی کم پروتئین حاملگی
کلمات کلیدی
برنامه ریزی بیهوشی، عدم تحمل گلوکز، مقاومت به انسولین، دیابت نابالغ، دیابت نوع 2،
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی پزشکی و دندانپزشکی (عمومی)
چکیده انگلیسی

BackgroundType 2 diabetes (T2D) in lean individuals is not well studied and up to 26% of diabetes occurs in these individuals. Although the cause is not well understood, it has been primarily attributed to nutritional issues during early development.ObjectiveOur objective was to develop a lean T2D model using gestational low-protein (LP) programming.Study DesignPregnant rats were fed control (20% protein) or isocaloric LP (6%) diet from gestational day 4 until delivery. Standard diet was given to dams after delivery and to pups after weaning. Glucose tolerance test was done at 2, 4, and 6 months of age. Magnetic resonance imaging of body fat for females was done at 4 months. Rats were sacrificed at 4 and 8 months of age and their perigonadal, perirenal, inguinal, and brown fat were weighed and expressed relative to their body weight. Euglycemic-hyperinsulinemic clamp was done around 6 months of age.ResultsMale and female offspring exposed to a LP diet during gestation developed glucose intolerance and insulin resistance (IR). Further, glucose intolerance progressed with increasing age and occurred earlier and was more severe in females when compared to males. Euglycemic-hyperinsulinemic clamp showed whole body IR in both sexes, with females demonstrating increased IR compared to males. LP females showed a 4.5-fold increase in IR while males showed a 2.5-fold increase when compared to their respective controls. Data from magnetic resonance imaging on female offspring showed no difference in the subcutaneous, inguinal, and visceral fat content. We were able to validate this observation by sacrificing the rats at 4 and 8 months and measuring total body fat content. This showed no differences in body fat content between control and LP offspring in either males or females. Additionally, diabetic rats had a similar body mass index to that of the controls.ConclusionLP gestational programming produces a progressively worsening T2D model in rats with a lean phenotype without obesity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: American Journal of Obstetrics and Gynecology - Volume 214, Issue 4, April 2016, Pages 540.e1-540.e7
نویسندگان
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