کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6147482 1594962 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Gardnerella vaginalis comprises three distinct genotypes of which only two produce sialidase
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی پزشکی و دندانپزشکی (عمومی)
پیش نمایش صفحه اول مقاله
Gardnerella vaginalis comprises three distinct genotypes of which only two produce sialidase
چکیده انگلیسی

ObjectiveSialidase and the presence of Gardnerella vaginalis have been proposed as biomarkers for bacterial vaginosis. Sialidase has been associated with adverse pregnancy outcome. We genotyped G vaginalis isolates, assessed the presence and diversity of sialidase-encoding genes, and determined the production of sialidase.Study DesignOne hundred thirty-four G vaginalis isolates were genotyped by random amplified polymorphic deoxyribonucleic acid (RAPD) and a selection of 29 isolates with amplified ribosomal deoxyribonucleic acid restriction analysis (ARDRA). A G vaginalis sialidase quantitative polymerase chain reaction was developed, and the sialidase production was assessed with the filter spot test.ResultsThree G vaginalis genotypes could be distinguished by both RAPD and ARDRA. Only 2 genotypes encoded and produced sialidase.ConclusionThree genotypes exist among G vaginalis isolates, and there is a clear link between genotype and sialidase production. A possible link between sialidase production and (symptomatic) bacterial vaginosis and biofilm production can be hypothesized.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: American Journal of Obstetrics and Gynecology - Volume 204, Issue 5, May 2011, Pages 450.e1-450.e7
نویسندگان
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