کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6162596 1249400 2012 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Laser microdissection and mass spectrometry-based proteomics aids the diagnosis and typing of renal amyloidosis
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی بیماری‌های کلیوی
پیش نمایش صفحه اول مقاله
Laser microdissection and mass spectrometry-based proteomics aids the diagnosis and typing of renal amyloidosis
چکیده انگلیسی
Accurate diagnosis and typing of renal amyloidosis is critical for prognosis, genetic counseling, and treatment. Laser microdissection and mass spectrometry are emerging techniques for the analysis and diagnosis of many renal diseases. Here we present the results of laser microdissection and mass spectrometry performed on 127 cases of renal amyloidosis during 2008-2010. We found the following proteins in the amyloid deposits: immunoglobulin light and heavy chains, secondary reactive serum amyloid A protein, leukocyte cell-derived chemotaxin-2, fibrinogen-α chain, transthyretin, apolipoprotein A-I and A-IV, gelsolin, and β-2 microglobulin. Thus, laser microdissection of affected areas within the kidney followed by mass spectrometry provides a direct test of the composition of the deposit and forms a useful ancillary technique for the accurate diagnosis and typing of renal amyloidosis in a single procedure.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Kidney International - Volume 82, Issue 2, 2 July 2012, Pages 226-234
نویسندگان
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