کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6178781 1253285 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Simultaneous assessment of aneuploidy, polymorphisms, and mitochondrial DNA content in human polar bodies and embryos with the use of a novel microarray platform
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی زنان، زایمان و بهداشت زنان
پیش نمایش صفحه اول مقاله
Simultaneous assessment of aneuploidy, polymorphisms, and mitochondrial DNA content in human polar bodies and embryos with the use of a novel microarray platform
چکیده انگلیسی

ObjectiveTo develop a microarray platform that allows simultaneous assessment of aneuploidy and quantification of mitochondrial DNA (mtDNA) in human polar bodies and embryos.DesignOptimization and validation applied to cell lines and clinical samples (polar bodies, blastomeres, and trophectoderm biopsies).SettingUniversity research laboratory and a preimplantation genetic diagnosis (PGD) reference laboratory.Patient(s)Samples from 65 couples who underwent PGD for aneuploidy and/or a single-gene disorder.Intervention(s)None.Main Outcome Measure(s)1) Comparison of aneuploidy screening results obtained with the use of the new microarray with those derived from two well established cytogenetic techniques. 2) mtDNA quantification. 3) Analysis of single-nucleotide polymorphisms.Result(s)The fully optimized microarray was estimated to have an accuracy of ≥97% for the detection of individual aneuploidies and to detect 99% of chromosomally abnormal embryos. The microarray was shown to accurately determine relative quantities of mtDNA. Information provided from polymorphic loci was sufficient to allow confirmation that an embryo was derived from specific parents.Conclusion(s)It is hoped that methods such as those reported here, which provide information on several aspects of oocyte/embryo genetics, could lead to improved strategies for identifying viable embryos, thereby increasing the likelihood of successful implantation. Additionally, the provision of genotyping information has the potential to reveal DNA contaminants and confirm parental origin of embryos.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fertility and Sterility - Volume 102, Issue 5, November 2014, Pages 1385-1392
نویسندگان
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