کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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6180497 | 1253466 | 2012 | 11 صفحه PDF | دانلود رایگان |
ObjectiveTo study the regulation of apoptosis in human endometrial cells. The specific aims were to determine whether milk fat globule-epidermal growth factor 8 (MFG-E8), a novel endometrial epithelial protein, modulates caspase activation and DNA fragmentation; and to examine whether hCG, an early embryonic product, regulates Bax and Bcl-2 equilibrium, as well as MFG-E8 expression.DesignPrimary cultures of human endometrial epithelial cells (EECs) and endometrial stromal cells (ESCs).SettingAcademic center.Patient(s)Ovulatory women aged 21-30 years.Intervention(s)Treatment with MFG-E8 and hCG.Main Outcome Measure(s)Apoptotic activity was quantified using a luciferase assay. Deoxyribonucleic acid fragmentation was detected by TUNEL assay. Bax, Bcl-2, and MFG-E8 messenger RNA expression levels were determined by quantitative reverse transcription-polymerase chain reaction. Immunocytochemistry was used to establish cell purity and presence of MFG-E8 and hCG-R (receptor) proteins.Result(s)Endometrial epithelial cells were cytokeratin+, vimentinâ, MFG-E8+, and hCG-R+, whereas ESC were vimentin+, cytokeratinâ, MFG-E8â, and hCG-R+. Treatment of ESC with MFG-E8 resulted in a 13-fold increase in caspase activity and a 30-fold increase in TUNEL. On the other hand, hCG decreased messenger RNA expression of Bax in ESC.Conclusion(s)Milk fat globule-epidermal growth factor 8 has proapoptotic activity, suggesting participation in endometrial remodeling via an epithelial-stromal cell paracrine effect. Conversely, pregnancy levels of hCG has opposite effects on stromal cells.
Journal: Fertility and Sterility - Volume 98, Issue 6, December 2012, Pages 1549-1556.e3