کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6196331 1602576 2016 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Preliminary investigation of multispectral retinal tissue oximetry mapping using a hyperspectral retinal camera
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی و میکروب شناسی (عمومی)
پیش نمایش صفحه اول مقاله
Preliminary investigation of multispectral retinal tissue oximetry mapping using a hyperspectral retinal camera
چکیده انگلیسی


- High spectral resolution multispectral fundus images measured under 3 s.
- Simple linear model yields relative oximetric and hemoglobin maps computed in 30 s.
- Relative oximetric maps computed automatically and without any a priori values.
- Method validated during systemic oximetry manipulations in healthy subjects.

Oximetry measurement of principal retinal vessels represents a first step towards understanding retinal metabolism, but the technique could be significantly enhanced by spectral imaging of the fundus outside of main vessels. In this study, a recently developed Hyperspectral Retinal Camera was used to measure relative oximetric (SatO2) and total hemoglobin (HbT) maps of the retina, outside of large vessels, in healthy volunteers at baseline (N = 7) and during systemic hypoxia (N = 11), as well as in patients with glaucoma (N = 2). Images of the retina, on a field of view of ∼30°, were acquired between 500 and 600 nm with 2 and 5 nm steps, in under 3 s. The reflectance spectrum from each pixel was fitted to a model having oxy- and deoxyhemoglobin as the main absorbers and scattering modeled by a power law, yielding estimates of relative SatO2 and HbT over the fundus. Average optic nerve head (ONH) saturation over 8 eyes was 68 ± 5%. During systemic hypoxia, mean ONH saturation decreased by 12.5% on average. Upon further development and validation, the relative SatO2 and HbT maps of microvasculature obtained with this imaging system could ultimately contribute to the diagnostic and management of diseases affecting the ONH and retina.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Eye Research - Volume 146, May 2016, Pages 330-340
نویسندگان
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