کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6196768 1602594 2014 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Experimental scleral cross-linking increases glaucoma damage in a mouse model
ترجمه فارسی عنوان
اتصال متقاطع اسکلرال تجربی سبب آسیب گلوکوم در یک مدل مو می شود
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی و میکروب شناسی (عمومی)
چکیده انگلیسی


- Glyceraldehyde (GA) exposure to eye had no effect on retinal structure, or function.
- GA injected eyes endured an increased cross-linking of sclera by 37%.
- GA injected eyes underwent a decrease of scleral permeability.
- GA injected eyes increased in the pressure-strain behavior by in-vitro testing.
- GA and glaucoma eyes had higher IOP and greater RGC loss vs. control and buffer eyes.

The purpose of this study was to assess the effect of a scleral cross-linking agent on susceptibility to glaucoma damage in a mouse model.CD1 mice underwent 3 subconjunctival injections of 0.5 M glyceraldehyde (GA) in 1 week, then had elevated intraocular pressure (IOP) induced by bead injection. Degree of cross-linking was measured by enzyme-linked immunosorbent assay (ELISA), scleral permeability was measured by fluorescence recovery after photobleaching (FRAP), and the mechanical effects of GA exposure were measured by inflation testing. Control mice had buffer injection or no injection in 2 separate glaucoma experiments. IOP was monitored by Tonolab and retinal ganglion cell (RGC) loss was measured by histological axon counting. To rule out undesirable effects of GA, we performed electroretinography and detailed histology of the retina. GA exposure had no detectable effects on RGC number, retinal structure or function either histologically or electrophysiologically. GA increased cross-linking of sclera by 37% in an ELISA assay, decreased scleral permeability (FRAP, p = 0.001), and produced a steeper pressure-strain behavior by in vitro inflation testing. In two experimental glaucoma experiments, GA-treated eyes had greater RGC axon loss from elevated IOP than either buffer-injected or control eyes, controlling for level of IOP exposure over time (p = 0.01, and 0.049, multivariable regression analyses). This is the first report that experimental alteration of the sclera, by cross-linking, increases susceptibility to RGC damage in mice.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Eye Research - Volume 128, November 2014, Pages 129-140
نویسندگان
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