کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6197160 1602603 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Sera from patients with seropositive neuromyelitis optica spectral disorders caused the degeneration of rodent optic nerve
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی و میکروب شناسی (عمومی)
پیش نمایش صفحه اول مقاله
Sera from patients with seropositive neuromyelitis optica spectral disorders caused the degeneration of rodent optic nerve
چکیده انگلیسی


- Autoantibody against AQP4 in serum from the patients with NMOSDs was assayed.
- The patients' sera were directly applied on rat optic nerve.
- The serum of AQP4+ group reduced AQP4 and GFAP expression in the optic nerve at 7 days.
- Marked inflammation was observed in the optic nerve treated with AQP4+ serum.
- Axonal damage and loss of retinal ganglion cells were confirmed at 14 days after the treatment.

Neuromyelitis optica (NMO) is an autoimmune inflammatory, neurodestructive disease primarily targeting the optic nerve and spinal cord. An autoantibody against water channel protein aquaporin-4 (AQP4), which is expressed at endofeet of astrocytes has been implicated in the pathogenesis of NMO. We evaluated the impact of sera of seropositive patients with NMO spectrum disorders (NMOSDs) on the rodent optic nerve and retina. Serum was obtained either from patients with seropositive NMOSD (AQP4+), seronegative patient with idiopathic optic neuritis (AQP4−), and healthy volunteers (control). Anti-AQP4 antibody in a serum was measured by a previously established cell-based assay. The patients' sera were applied on the optic nerve after de-sheathed. Immunohistochemistry showed that at 7 days after the treatment, the area of the optic nerve exposed to the AQP4+ sera lost expression of both AQP4 and glial fibrillary acidic protein. Also, Human-IgG immunoreactivity and marked invasion of inflammation cells were observed in the optic nerve treated with AQP4+ serum. Immnoreactivity of neurofilament was reduced at 14 days after the treatment, not 7 days. Real-time polymerase chain reaction revealed the reduced gene expression of neurofilament in retina from the eye that was exposed to the AQP4+ sera at 14 days. Retrograde fluorogold-labeling on the retinal flatmount disclosed the significantly reduced number of retinal ganglion cells when the AQP4+ sera were applied. The present model has demonstrated that the sera from patients with seropositive NMOSDs led to the regional astrocytic degeneration and inflammatory cell invasion in the optic nerve, resulting in the ultimate loss of RGCs and their axons at areas beyond the injury site.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Eye Research - Volume 119, February 2014, Pages 61-69
نویسندگان
, , , , , ,