کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6197623 | 1261200 | 2009 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Human iris pigment epithelium suppresses activation of bystander T cells via TGFβ-TGFβ receptor interaction Human iris pigment epithelium suppresses activation of bystander T cells via TGFβ-TGFβ receptor interaction](/preview/png/6197623.png)
Iris pigment epithelial (IPE) cells from the anterior segment in the eye are able to suppress activation of bystander responder T cells in vitro. The cultured IPE cells fully suppress proliferation and cytokine production by responder T cells via direct cell-to-cell contact. We have now investigated whether primary cultured human iris pigment epithelial (h-IPE) cells that were established from fresh iris tissues can also inhibit the activation of T cells in vitro. We found that cultured h-IPE cells significantly inhibited T cell proliferation and the IFN-γ production by the target T cells from both the allogeneic and autogeneic peripheral blood mononuclear cells (PBMCs). The h-IPE cells also inhibited the activation of CD4+ T cells from patients with active uveitis. The suppression by h-IPE occurred in a completely contact-dependent manner. The h-IPE constitutively expressed transforming growth factor β (TGFβ) and the receptors, and the T cells exposed to h-IPE greatly expressed Smad transcripts. In addition, TGFβ2-siRNA transfected h-IPE failed to inhibit activation of responder T cells. Similarly, h-IPE cells in the presence of anti-TGFβ neutralizing antibodies or recombinant TGFβ receptor blocking proteins failed to inhibit the T-cell activation. In conclusion, cultured human iris pigment epithelium fully inhibits T cell activation in vitro. Our data support the hypothesis that the ocular resident cells play a critical role in immunosuppression in the eye.
Journal: Experimental Eye Research - Volume 88, Issue 6, 1 June 2009, Pages 1033-1042